Diverse Effects of Environmentally Relevant Metal-based Nanoparticle and Pesticide Mixtures on Soil Fauna: A Novel Issue for Risk Assessment (DEFENSoil)
Croatian science foundation
Department of Biology
Department of Biotechnology
Institute Ruđer Bošković
Josip Juraj Strossmayer University
Subdepartment of quantitative ecology
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Published paper: Is nano ZnO/chlorpyrifos mixture more harmful to earthworms than bulk ZnO? A multigeneration approach
A paper was published in the journal Chemosphere (IF 5.108; Q1) titled: Is nano ZnO/chlorpyrifos mixture more harmful to earthworms than bulk ZnO? A multigeneration approach.
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Final report of the project
The final report of the DEFENSoil project can be downloaded by clicking at the link: Download
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Final presentation of the DEFENSoil project
The final presentation of our project was held on 31st October 2019. at the Department of Biology, Josip Juraj Strossmayer University of Osijek.
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A Master thesis
Tea Galinović, a student at the Department of Biology successfully defended her master thesis entitled: „Effect of silver nanoparticles size on biochemical biomarkers and reproduction of Enchytraeus albidus (Oligochaeta)“.
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Published paper: Observing earthworm behavior using deep learning
A paper was published in the journal Geoderma (IF: 4,336) titled: Observing earthworm behavior using deep learning.
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Participation in the ISEM Global Conference 2019.
Two members of the project team participated in The International Society for Ecological Modelling Global Conference 2019. held in Salzburg, Austria from 1st to 5th October 2019.
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Another Master thesis!
Student Luka Ivanković has successfully defended a master thesis titled: „The effects of propiconazole and chlorantraniliprole on terrestrial cyanobacterium Cylindrospermum sp.“.
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A Master thesis!
Marija Andabak has successfully defended a master thesis titled: „Metallothionein detection in polyacrylamide gel by electrophoresis“.
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A Master thesis!
Dora Bjedov has successfully defended a master thesis titled: „Effect of chlorpyrifos on molecular biomarkers in different parts of the body of earthworm Lumbricus terrestris L.“.
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Participation in the SEH 2019 20th European Congress
A member of our project team participated in the Societas Europaea Herpetologica 2019 20th European Congress held between 2nd and 6th September in Milan, Italy.
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Symposium Soil Ecotoxicology: Theory and Application held
The symposium entitled Soil Ecotoxicology: Theory and Application was successfully held on 27th June 2019.
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Participation in the 29. SETAC Europe congress
Four members of the project team successfully participated in the 29th SETAC Europe Annual Meeting held in Helsinki, 26-30 May, with four presentations
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Invitation to Symposium: Soil Ecotoxicology: Theory and Application, Osijek, 27th June 2019.
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Experimental field established
A plot of land was established for field experiments.
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Published paper: Effects of single and combined exposure to nano and bulk zinc-oxide and propiconazole on Enchytraeus albidus
A paper was published in the journal Chemosphere (IF: 4,427) under the title “Effects of single and combined exposure to nano and bulk zinc-oxide and propiconazole on Enchytraeus albidus”.
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Published paper: Real-time CO2 uptake/emission measurements as a tool for early indication of toxicity in Lemna-tests
In the journal Aquatic Toxicology (IF: 3,884) a paper titled “Real-time CO2 uptake/emission measurements as a tool for early indication of toxicity in Lemna-tests” was published.
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EUROTOX specialized toxicology course 2018
Our PhD student, Marija Kovačević, attended the EUROTOX specialized toxicology course 2018 - Regulatory toxicology, held in Slovenia from 4th to 10th November this year.
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Published paper: Biochemical and reproductive effects of red mud to earthworm Eisenia fetida
The paper “Biochemical and reproductive effects of red mud to earthworm Eisenia fetida” was published in the journal Ecotoxicology and Environmental Safety (IF: 3,974) in collaboration with dr. sc. Viktoria Feigl, a colleague from Budapest.
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New equipment - HAVER EML 200 Premium Remote
A HAVER EML 200 Premium Remote sieve shaker was purchased with funds provided by Croatian science foundation, allowing wet sieving analysis of soil samples.
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PhD student employed on the DEFENSoil project
Within the young scientists’ career development program of the Croatian science foundation - training of new PhDs - Marija Kovačević, mag.biol. was employed. Through her research she will contribute to the success of the DEFENSoil project.
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Participation in the 1st International Earthworm Congress
Two members of the project team had participated in the 1st International Earthworm Congress in Shanghai from June 24-29 2018. Research conducted within the DEFENSoil project had been presented with an oral and a poster presentation.
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A Master thesis!
Student Nikola Čurčić has successfully defended a master thesis titled: „Single and combined effects of propiconazole and ZnO (bulk and nano form) on various biomarkers and reproduction in Enchytraeus albidus“
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Measuring the metabolic activity of earthworms
Laboratory measurements of the metabolic activity of earthworms are carried out using the ResTox device constructed within the DEFENSoil project.
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Gilson pipettese – pipetman M and Distriman
Gilson pipettes (M multi and Distriman) were purchased with funds provided by Croatian science foundation.
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Published paper: Acute and subchronic effects of three herbicides on biomarkers and reproduction in earthworm Dendrobaena veneta
The paper “Acute and subchronic effects of three herbicides on biomarkers and reproduction in earthworm Dendrobaena veneta” has been published in the journal “Chemosphere” (IF 4,427).
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Participation in the 28. SETAC Europe congress
Five members of the project team had successfully participated in the 28. SETAC congress in Rome from May 13-17 2018 with four presentations
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Participation in meeting: 1st International Students’ GREEN Conference
Studentis Marija Andabak, Ivana Živković i Marija Paurević, with the guidance of Martine Šrajer Gajdošik, participated in the 1st International Students’ GREEN Conference with a poster presentation titled: The role of metallothioneins in detoxification and oxidative stress
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An article in the University journal
An article about the development of the ResTox metabolic activity measuring system was published in Glas Slavonije’s university journal.
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Guest researcher
A cheerful laboratory work with our guest researcher Salsabil Trigui from Tunisia!
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Mesocosms
As scheduled for the third year of our project, the mesocosms were set up equipped with sensor systems.
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New equipment – OMNI TH
An OMNI TH homogenizer was purchased with funds provided by Croatian science foundation. The homogenizer is characterized by a power of 150 W, allowing processing of tissue samples of volumes ranging between 0.2 and 100 mL.
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Reproduction test with earthworms D. veneta
Removing the juveniles of D. veneta following the conducted reproduction test.
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Prior to setting up the mesoscosms, micrososms were prepared containing members of various groups of experimental organisms (earthworms, Collembola, Enchytreidae) in artificial soil.
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Counting juvenile E. albidus
Counting juveniles of E. albidus after a cunducted reproduction test, according to the OECD protocol (OECD, 2004).
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Published paper: Influence of soil temperature and moisture on biochemical biomarkers in earthworm and microbial activity after exposure to propiconazole and chlorantraniliprole
The paper “Influence of soil temperature and moisture on biochemical biomarkers in earthworm and microbial activity after exposure to propiconazole and chlorantraniliprole” has been published in journal “Ecotoxicology and Environmental Safety”.
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Yet another Master thesis!
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At the Department of Biotechnology, University of Rijeka a master thesis -
Another Master thesis has been defended!
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Student Nikolina Stjepanović has successfully defended a master thesis titled: “The influence of herbicides on biomarkers of earthworm species Dendrobaena veneta (Rosa 1886)”. -
Sensor systems are assembled...
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One of the goals of DEFENSoil -
Bachelor’s thesis by student Ana Oršolić
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A bachelor’s thesis by student Ana Oršolić titled: “Differentiation of biochemical markers of exposure to pesticides and nanoparticles in enchytraeids” -
A new equipment has arrived!!!
The climate chamber for enhancing the capacities for culturing of model organisms
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An annual meeting of the project team was held
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An annual meeting of the project team was held at the Department of Biotechnology in Rijeka -
Participation in the 27. SETAC congress
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Two members of the project team have successfully participated in the SETAC congress in Brussels from May 7-11 2017. with two presentations -
A Master thesis has been defended within the project DEFENSoil
A Master thesis titled: Impact of zinc nanoparticles on the expression of biochemical markers of exposure in earthworm Eisenia fetida has been defended within the project DEFENSoil
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An article in the University journal
An article about our project was published in Glas Slavonije’s university journal. Click here to download the article
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Participation in Crotox congress --
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Branimir K. Hackenbeger, our project manager has participated in the 5th Croatian Congress of Toxicology with international participation (Crotox) in Poreč, where he presented the DEFENSoil project within his invited lecture “COMPUTING ECOTOXICOLOGY – ACTUALITY AND PERSPECTIVES”. -
Selecting appropriate locations and first soil sampling
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We were on field to select appropriate locations and carry out the first soil sampling. -
New equipment
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Laboratory centrifuge has arrived purchased with funds provided by Croatian science foundation! -
Kickoff meeting
A kickoff meeting was held where we arranged directives for the first project period.
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